|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Delivery Time:||6 days|
|Supply Ability:||1000000g per week|
|Product:||Recombinant Porcine Trypsin||Cat. No.:||RPT0201|
|Contaminant Activity:||No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.||Additives:||Additives|
|Related Product:||Recombinant Human Trypsin||Source:||Recombinant E.coli|
Recombinant Trypsin Manufacturer USP Trypsin Supplier USA Trypsin
Enzyme Commission (EC) Number: 184.108.40.206
Molecular Weight: 23.3 kDa (bovine & porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5 (bovine)
Pipette (in milliliters) the following reagents into suitable quartz cuvettes:
|Reagent B (BAEE)||3.00||3.00|
Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted
|Reagent C (HCl)||—||0.20|
|Reagent D (Enzyme Solution)||0.20||—|
Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes.
Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.
|Physical form||White lyophilized powder|
|Specific activity||≥3800 USP units/mg pro|
|Purity(RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
|Contaminant activity||No chymotrypsin, carboxypeptidase A, and other protease contaminant.|
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute
in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases
the chance of activity loss in the process of transport and storage.
Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Contact Person: Miss Eland