|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packing|
|Supply Ability:||1000g per week|
|Product:||Recombinant Porcine Trypsin||Cat. No.:||RPT0201|
|Specific Activity:||≥3800 USP U/mg Pro||Purity:||> 99%|
|Contaminant Activity:||No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.||Related Product:||Recombinant Human Trypsin|
Cell Dissociation Solution Recombinant Trypsin in E.coli for Cell Fermentation
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.
Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.
Defined, animal component-free
|Pure, recombinant trypsin - no contaminating proteases|
|Optimized for sensitive cells, such as hMSCs|
|Compatible with cells cultured in both serum-free and serum-containing media|
Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
Recombinant trypsin solution is a pure enzyme solution, helps to maximize the yield of functionally viable cells from culture vessels, while preventing the toxicity effect induced by other non desirable proteases. In addition, Rec. Trypsin eliminates the risk of viruses, or other potential adventitious agents found in animal derived solutions. This special animal component free, recombinant Trypsin solution, is an alternative for bulk production of porcine trypsin . Rec.Trypsin is produced by submerged fermentation of Fusarium oxysporum. It is derived from a production process which does not utilize any raw materials and/or processing aids of animal origin.
Contact Person: Miss Eland