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Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

  • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
  • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
  • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
  • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 65$/10mg
Packaging Details: ice packaging, carton
Delivery Time: 6 days
Payment Terms: T/T
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Porcine Trypsin Cat. No.: RPT0201
Physical Form: White Or White-like Lyophilized Powder Additives: Carbohydrates
Protein Content: 35% ~ 70% Related Product: Recombinant Trypsin Solution
High Light:

porcine trypsin

,

trypsin enzyme

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
 
Recombinant Trypsin
Description
 
Trypsin
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(porcine)

Trypsinogen
Molecular Weight: 24 kDa (porcine)
Extinction Coefficient: E1% = 14.4 (280 nm)
pI: 9.3 (porcine)

Procedure

Pipette (in milliliters) the following reagents into suitable quartz cuvettes:

 TestBlank
Reagent B (BAEE)3.003.00

 

 

 

Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted spectrophotometer. Then add:

 TestBlank
Reagent C (HCl)----0.20
Reagent D (Enzyme Solution)0.20----

 

 

 

 

Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes. Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.

 

Calculation

 

BAEE Units/ml enzyme =(ΔA253nm/min Test - ΔA253nm/min Blank)(df)
(0.001) (0.20)

 

 

 

df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction mix
0.20 = Volume (in milliliters) of enzyme used

Units/mg solid=units/ml enzyme
mg solid/ml enzyme

 
 
 
Advantages
 
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. 
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
 
Application
Use Trypsin recombinant, Proteomics Grade, to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.
 
Recommend Usage
 
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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