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Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

    • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
    • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
    • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
    • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
  • Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001:2015
    Model Number: RPT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 65$/10mg
    Packaging Details: ice packaging, carton
    Delivery Time: 6 days
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Recombinant Porcine Trypsin Cat. No.: RPT0201
    Physical Form: White Or White-like Lyophilized Powder Additives: Carbohydrates
    Protein Content: 35% ~ 70% Related Product: Recombinant Trypsin Solution
    High Light:

    porcine trypsin

    ,

    trypsin enzyme

    Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
     
    Recombinant Trypsin
    Description
     
    Trypsin
    Enzyme Commission (EC) Number: 3.4.21.4
    Molecular Weight: 23.3 kDa (porcine)
    Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
    pI: 10.1 - 10.5(porcine)

    Trypsinogen
    Molecular Weight: 24 kDa (porcine)
    Extinction Coefficient: E1% = 14.4 (280 nm)
    pI: 9.3 (porcine)

    Procedure

    Pipette (in milliliters) the following reagents into suitable quartz cuvettes:

     TestBlank
    Reagent B (BAEE)3.003.00

     

     

     

    Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted spectrophotometer. Then add:

     TestBlank
    Reagent C (HCl)----0.20
    Reagent D (Enzyme Solution)0.20----

     

     

     

     

    Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes. Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.

     

    Calculation

     

    BAEE Units/ml enzyme =(ΔA253nm/min Test - ΔA253nm/min Blank)(df)
    (0.001) (0.20)

     

     

     

    df = Dilution factor
    0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction mix
    0.20 = Volume (in milliliters) of enzyme used

    Units/mg solid=units/ml enzyme
    mg solid/ml enzyme

     
     
     
    Advantages
     
    Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. 
    Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
    High purity:
    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
     
    Application
    Use Trypsin recombinant, Proteomics Grade, to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.
     
    Recommend Usage
     
    Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
    Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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