Place of Origin: | China |
Brand Name: | YaxinBio |
Certification: | NSF ISO 9001 2015 |
Model Number: | RCT10 |
Minimum Order Quantity: | 10mg |
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Price: | 89$/10mg |
Packaging Details: | ice packaging |
Payment Terms: | T/T |
Supply Ability: | 1000g per week |
Packaging: | 10mg,100mg,1g Or Bulk | Source: | E. Coli |
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CAS: | 9004-07-3 | EC: | 3.4.21.1 |
Specific Activity: | ≥1000 Unit/mg Pro | Purified By: | HPLC |
Chymotrypsin Enzyme lyophilized Powder Human Chymotrypsin for Digest Protein
Description
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC,
the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes
at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be
observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
Advantages
Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and
other potential adventitious agents found in animal-derived chymotrypsin.
High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated
other proteases activities found in enzymes purified from pancreas.
Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.
Main Features
M.W. |
26,950 Da |
Purified by |
HPLC |
Format |
White lyophilized |
Purity |
> 95% by HPLC |
Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
Recommend Usage
Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored below -20℃ after repacked
Storage Instructions
Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃in sealed container.It is stable within 24 months. After dissolved, it should be stored under -20℃.
Application
Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for
peptide mapping, fingerprinting, and sequence analysis.
Contact Person: Miss Eland