Chymotrypsin Enzyme lyophilized Powder Human Chymotrypsin for Digest Protein

Chymotrypsin Enzyme lyophilized Powder Human Chymotrypsin for Digest Protein

Description

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC,

the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes

at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be

observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

Advantages

Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and

other potential adventitious agents found in animal-derived chymotrypsin.

High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated

other proteases activities found in enzymes purified from pancreas.

Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.       

Main Features

Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Recommend Usage

Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored below -20℃ after repacked

Storage Instructions

Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃in sealed container.It is stable within 24 months. After dissolved, it should be stored under -20℃.

Application

Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for

peptide mapping, fingerprinting, and sequence analysis.