|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Supply Ability:||1000g per week|
|Source :||E. Coli||Purified by:||HPLC|
|RELATED PRODUCT :||V8||Cat. No.:||RCT10|
Recombinant Enzyme Human Chymotrypsin for Digest Protein CAS 9004-07-3
Recombinant human chymotrypsin
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥1000 USP units/mg pro.|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||24.0±2.4 kDa|
UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.
Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.
High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.
Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.
Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.
White lyophilized r
≥1000 unit/mg pro
> 95% by HPLC
Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved wth 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored below -20℃ after repacked
Contact Person: Miss Eland