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Recombinant Enzyme Human Chymotrypsin for Digest Protein CAS 9004-07-3

Recombinant Enzyme Human Chymotrypsin for Digest Protein CAS 9004-07-3

    • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3
    • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3
    • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3
    • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3
    • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3
  • Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 89$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Source  : E. Coli Purified by: HPLC
    RELATED PRODUCT : V8 Cat. No.: RCT10
    Product: Recombinant Chymotrypsin Manufacturer: YaxinBio
    CAS: 9004-07-3

    Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3

     

    Recombinant human chymotrypsin

     

    DESCRIPTION

    Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

     

     

    MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white powder
    Specific activity ≥1000 USP units/mg pro.
    SDS-PAGE Single major band
    SDS-PAGE (MW) 24.0±2.4 kDa

    UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.

     

     

    Advantages

    Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

    High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.

    Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.       

     

     

    Application

    Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.

     

     

    Main Features

    M.W.

    26,950 Da  

    Format

    White lyophilized r

    Specific activity

    ≥1000 unit/mg pro

    Purity

    > 95% by HPLC

     

     

     

     

     

    Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

     

     

    STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

     

    Recommend Usage

    Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored below -20℃ after repacked

     

    Recombinant Enzyme  Human Chymotrypsin for Digest Protein  CAS 9004-07-3

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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