|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||ice packaging|
|Delivery Time:||7 work days|
|Supply Ability:||100000g per week|
|Source :||E. Coli||Purified by:||HPLC|
|Physical form :||White Or White-like Lyophilized Powder||Additives :||Tris Salts, NaCl Salts And Carbohydrates|
|Protein content :||35% ～ 50%||Purity :||> 95%|
|Nature:||Excision Enzymes||Advantage:||Animal Component Free|
Recombinant Carboxypeptidase B is an excision enzymes with Animal Components Free
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved wth sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
The production of recombinant insulin and its analogues;
the quality test of recombinant antibodies;
the determination of C-terminal amino acids of proteins;
the production of other recombinant polypeptides;
and the enzymatic synthesis of certain specific compounds.
Contact Person: Miss Eland