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Recombinant Carboxypeptidase B is an excision enzymes with Animal Components Free

Recombinant Carboxypeptidase B is an excision enzymes with Animal Components Free

    • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free
    • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free
    • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free
    • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free
    • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free
  • Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Delivery Time: 7 work days
    Payment Terms: T/T
    Supply Ability: 100000g per week
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    Detailed Product Description
    Source  : E. Coli Purified by: HPLC
    Physical form : White Or White-like Lyophilized Powder Additives : Tris Salts, NaCl Salts And Carbohydrates
    Protein content  : 35% ~ 50% Purity   : > 95%
    Nature: Excision Enzymes Advantage: Animal Component Free

    Recombinant Carboxypeptidase B is an excision enzymes with Animal Components Free

     

     

     

    Abbr :CPB1, Recombinant (Rat)
    Alias:CPB1
    Source:E. coli
    Species:Rat
    Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl.
    Enzyme Commission Number:3.4.17.2
    CAS No.:9025-24-5
     
     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

     

    Applications

     

    Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.
     
     

    STABILITY OF STORAGE AND TRANSPORT

     

    Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.

    It is no activity loss after 10 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    PRODUCT USAGE

    The production of recombinant insulin and its analogues;

    the quality test of recombinant antibodies;

    the determination of C-terminal amino acids of proteins;

    the production of other recombinant polypeptides;

    and the enzymatic synthesis of certain specific compounds.

     

    Recombinant Carboxypeptidase B is an  excision enzymes  with Animal Components Free

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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