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Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

    • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade
    • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade
    • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade
    • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade
    • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade
  • Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Brand: YaxinBio
    Protein Content: 35% ~ 50% Specific Activity: ≥170 Units/mg Pro
    Purity: > 95% Cat. No.: RCPB01

    Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

     

    CAS: 9025-24-5
    EC: 3.4.17.2

    Source: Expressed in E. Coli.

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

    Main Features

     

    Source E. Coli
    Purified by HPLC
    Format White or white-like or yellowish lyophilized
    Additives Tris salts, NaCl salts and carbohydrates
    Purity Single main band on SDS-PAGE
    M.W. 33.8kD
    Contaminant activity Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

     

     

    Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    Advantages

     

    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.

     

     

    Recommend Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     

    Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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