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Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.

Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.

    • Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.
    • Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.
    • Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.
    • Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.
  • Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 67$/100mg
    Packaging Details: ice packaging
    Delivery Time: 7 work days
    Payment Terms: T/T
    Supply Ability: 100g per week
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    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
    Source : E. Coli Purified by: HPLC
    Protein conten: 35% ~ 50% Contaminant activity: No Chymotrypsin,carboxypeptidase A,and Other Protease Contaminant.Less Than 10ppm Of Recombinant Trypsin.

    Recombinant Enzyme Carboxypeptidase B For Sale with purity 170 u / mg Pro

     

    Species:Rat
    Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl.
    Enzyme Commission Number: 3.4.17.2
    Bio-activity:50-55 units/mg protein carboxypeptidase B
    CAS No.:9025-24-5

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

     

    Advantages

     

    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

     

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

     

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.

     

    Main Features

     

    Format

    White or white-like or yellowish lyophilized

    Additives

    Tris salts, NaCl salts and carbohydrates

    Specific activity

     ≥170 units/mg pro.

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

     

    Unit Definition

     

    One Unit of carboxypeptidase B activity hydrolyzes one micromole of  hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    Recommend Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     
    Applications
     
    Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.
     

    Recombinant Enzyme Carboxypeptidase B for Sale with purity 170 u / mg pro.

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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