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Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein

Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein

    • Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein
    • Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein
    • Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein
    • Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein
  • Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
    Specific activity: ≥170 U/mg Pro Additives: Tris Salts, NaCl Salts And Carbohydrates
    Protein content: 35% ~ 60% Purity: > 95%

    Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein

     

     

    DESCRIPTION

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

     

    Main Features

     

    Source

    E. Coli

    Purified by

    HPLC

    Specific activity

     ≥170 units/mg pro.

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

    Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    Recommend Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    STABILITY OF STORAGE AND TRANSPORT

     

    Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.

    It is no activity loss after 10 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     

    Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting Protein

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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