Home ProductsRecombinant Carboxypeptidase B

YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

    • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
    • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
    • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
    • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
    • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 175$/100mg
    Packaging Details: ice packaging
    Delivery Time: 7 work days
    Payment Terms: T/T
    Supply Ability: 100000g per year
    Contact Now
    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
    Specific Activity: ≥170 U/mg Pro Physical Form: White Or White-like Lyophilized Powder
    Additives: Tris Salts, NaCl Salts And Carbohydrates Protein Content: 35% ~ 50%

    YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

     

    Recombinant Carboxypeptidase B

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

     

    Main Features

     

    Source

    E. Coli

    Purified by

    HPLC

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

    Unit  Definition

     

    One Unit of carboxypeptidase B activity hydrolyzes one micromole of  hippuryl-L-arginine per minute at 25℃, pH 7.65.

     
    Applications
     
    Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

     

    Recommend  Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     
    Pathway
     
    Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Protein digestion and absorption, organism-specific biosystem
     
    Function
     
    carboxypeptidase activity;
    metallocarboxypeptidase activity;
    zinc ion binding
    YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

    Send your inquiry directly to us (0 / 3000)

    Best Products
    Other Products