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Recombinant Carboxypeptidase B E.coli Determine C-terminal Amino Acids

Recombinant Carboxypeptidase B E.coli Determine C-terminal Amino Acids

    • Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids
    • Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids
    • Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids
    • Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids
  • Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Packaging: 10mg,100mg,1g,bulk
    Source: E. Coli Purified By: HPLC
    Specific Activity: ≥170 U/mg Pro Contaminant Activity: No Chymotrypsin,carboxypeptidase A,and Other Protease Contaminant. Less Than 10 Ppm Of Recombinant Trypsin.

    Recombinant Carboxypeptidase B E.coli   Determine C-terminal Amino Acids

     

    CAS: 9025-24-5
    EC: 3.4.17.2

    Source: Expressed in E. Coli.

     

    Description

     

    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

     

     

    Advantages

     

    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

     

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.

     

    Main Features

     

    Format

    White or white-like or yellowish lyophilized

    Additives

    Tris salts, NaCl salts and carbohydrates

    Protein content

    35% ~ 60%

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

     

    Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

     

    RECOMMEND USAGE

    Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Recombinant Carboxypeptidase B  E.coli   Determine C-terminal Amino Acids

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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