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Stable Recombinant Carboxypeptidase B with E.coli Carboxypeptidase B E.coli

Stable Recombinant Carboxypeptidase B with E.coli Carboxypeptidase B E.coli

    • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli
    • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli
    • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli
    • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli
    • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli
  • Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Delivery Time: 7 work days
    Payment Terms: T/T
    Supply Ability: 100000g per year
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    Detailed Product Description
    Product: Recombinant Carboxypeptidase B Manufacturer: YaxinBio
    Packaging: 10mg,100mg,1g,bulk Source: E. Coli
    Purified By: HPLC Purity: > 95%

    Stable Recombinant Carboxypeptidase B with E.coli  Carboxypeptidase B E.coli

     

    peptidyl-L-lysine [L-arginine]hydrolase
     
    EC :3.4.17.2
     
    CAS:9025-24-5
     
    Synonyms:carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B;
     
    Description
     
    Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 33.8 kDa. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
     
     

    Advantages

     

    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

     

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

     

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.

     

    Main Features

     

    Format

    White or white-like or yellowish lyophilized

    Additives

    Tris salts, NaCl salts and carbohydrates

    Protein content

    35% ~ 60%

    Specific activity

     ≥170 units/mg pro.

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

     

     

    Unit Definition

     

    One Unit of carboxypeptidase B activity hydrolyzes one micromole of  hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    Recommend Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

     

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    Stable Recombinant Carboxypeptidase B with E.coli    Carboxypeptidase B  E.coli

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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