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Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme with 170u / mg Pro

Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme with 170u / mg Pro

    • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro
    • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro
    • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro
    • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro
    • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro
  • Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: RCPB01

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 150$/10mg
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000 per week
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    Detailed Product Description
    Purified By: HPLC Physical Form: White Or White-like Lyophilized Powder
    Additives: Tris Salts, NaCl Salts And Carbohydrates Cat. No.: RCPB01
    Specific Activity: ≥170 U/mg Pro Purity: > 95%
    Impact: Mass Spectrometry

    Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme with 170u / mg Pro

     

     

    Description

     

    Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,

    and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited

    by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA,1,10-phenanthroline.

    Synonyms: Carboxypeptidase B, Cpb1, Cpb.

     

    Advantages

     

    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.

     

     

    Main Features

     

    Source

    E. Coli

    Protein content

    35% ~ 60%

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.

     

     

     

     

     

     

     

    Unit Definition

     

    One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

     

    Recommend Usage

     

    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

     

    Storage Instructions

     

    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Carboxypeptidase B for Mass Spectrometry Recombinant  Enzyme with 170u / mg Pro

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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