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Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.

Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.

  • Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
  • Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
  • Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
  • Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
  • Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 150$/10mg
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Purified By: HPLC Physical Form: White Or White-like Lyophilized Powder
Additives: Tris Salts, NaCl Salts And Carbohydrates Cat. No.: RCPB01
Specific Activity: ≥170 U/mg Pro Purity: ≥95%
Impact: Mass Spectrometry
High Light:

sequencing grade modified trypsin

,

trypsin endopeptidase

Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro.

 

Recombinant Carboxypeptidase B

 

Description

 

Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,

and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited

by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA,1,10-phenanthroline.

Synonyms: Carboxypeptidase B, Cpb1, Cpb.

 

Advantages

 

Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.

 

 

Main Features

 

Source

E. Coli

Protein content

35% ~ 60%

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.

 

 

 

 

 

 

 

Unit Definition

 

One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

 

Recommend Usage

 

To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

Carboxypeptidase B, For Mass Spectrometry, Recombinant Enzyme, Specific Activity 170u/mg Pro. 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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