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Enzyme Markers Monoclonal and Polyclonal Antibody Purification

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

    • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
    • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
    • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
    • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
    • Enzyme Markers Monoclonal and Polyclonal Antibody Purification
  • Enzyme Markers Monoclonal and Polyclonal Antibody Purification

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: RSPA05

    Payment & Shipping Terms:

    Minimum Order Quantity: 10mg
    Price: 79$/10mg
    Packaging Details: ice packaging
    Delivery Time: 5-8 weekdays
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Substrate: Porous Polystyrene-divinylbenzene Particle Size: 40 Um
    Ligand: Recombinant Protein A Recommend Flow Rate: 300-700 Cm/h
    DBC: At Least 300 Mg IgG/mL Media At 500 Cm/h Maximum Pressure: 1500 Psi
    Brand: Yaxinbio

    Enzyme Markers Monoclonal and Polyclonal Antibody Purification

     

    Recombinant Protein A

     

    Catalog Number:RSPA05

    CAS:91932-65-9

    Storage temperature: 2-8℃

    Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli

     

    1.DESCRIPTION

    Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.

     

    2.MAIN FEATURES

    Appearance White or off white powder
    Solubility Clear liquid
    Bioburden ≤ 1 cfu/ mg
    SDS-PAGE Single major band
    SDS-PAGE (MW) 25000±1000 Daltons
    UV Spectrum E275/250 ≥ 1.2
    E0.1% at 275nm = 0.17-0.23
    Purity (HPLC) ≥ 95%

     

    3.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

    After dissolved with pure water, it should be stored under -20℃.

    Stability of transport: The product is stable by blue ice insulation transport.

     

     

    4.APPLICATION:

    The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

     

    5.Product Specification

    Test/Method                    

    Specification

    Bioburden

    No organisms detected.

    12% SDS-PAGE

    Single major band

    Coomassie Stain

    25kD

    UV Spectrum

    E275/250 ≥1.2,

    E0.1% at 275nm=0.17-0.23 

    UV spectrometry

    No DNA or RNA detected

    Triton Content

    No Triton contaminant.

    Format

    Lyophilized

    pH stable range

    pH 1-1

     

     

     

     

     

     

     

     

     

     

     

     

     

    6.Storage Instructions

    Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

     

    7.Usage

    For Research or Manufacturing Purpose Only. Not for Human.

     

     

    8.RELATED PRODUCTS

    Recombinant carboxypeptidase B

    Sequencing grade trypsin

    Recombinant human trypsin

    Recombinant enterokinase

    Enzyme Markers Monoclonal and Polyclonal Antibody Purification

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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