|Place of Origin:||China|
|Certification:||ISO 9001 2015|
|Minimum Order Quantity:||10mg|
|Supply Ability:||1000g per week|
|Source:||E. Coli||Specific Activity:||≥1000 Unit/mg Pro|
|Physical Form:||White Lyophilized Powder||Purified By:||HPLC|
Human Chymotrypsin Proteolytic Enzyme Recombinant Enzyme CAS 9004-07-3 EC 184.108.40.206
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.
High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.
Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.
Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved wth 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis.
Contact Person: Miss Eland