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Shanghai Yaxin Biotechnology Co.,Ltd.

Sequencing Grade Trypsin purified by HPLC and the source of it is from E.coli

    • Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli
    • Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli
    • Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli
    • Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli
  • Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: ISO 9001 2015
    Model Number: SRT0201

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Price: 98$/100ug
    Packaging Details: ice packaging
    Delivery Time: 7 days
    Payment Terms: T/T
    Supply Ability: 1000g per week
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    Detailed Product Description
    Source: E. Coli Purified By: HPLC
    Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
    Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.

    Sequencing Grade Trypsin purified by HPLC and the source of it is from E.coli

     

     

    Description

     

    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

     

    Main Features

     

    Format

    Liquid in 50mM HAc, or fluffy solid.

    Protein concentration

    0.5mg/ml in 50mM HAc

    Purity(RP-HPLC)

    ≥99% by HPLC

    Contaminant activity

    Recombinant porcine trypsin.

    No any other proteases activities contaminant.

     

     

     

     

     

     

     

    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

     

    STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

    It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

    It is no activity loss after 5 times repeated freezing and thawing.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    Reconstitution

     

    Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

     

    Specificity

     

    Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

    Sequencing Grade Trypsin purified by HPLC and the source of it  is from E.coli

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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