|Place of Origin:||China, Shanghai, Xuhui|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||100U|
|Packaging Details:||100U, 500U, 1000U, 10KU, 100KU, 1MU; ice packaging, carton|
|Supply Ability:||1000ku per week|
|Source:||E. Coli||M.W.:||Theoretical MW: 25,850 Da;The Apparent MW On SDS-PAGE: About 27,000 Da|
|Storage:||Store At -20°C After Delivery.||Specific Activity:||One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.|
|Stability:||Keep Cool With Blue Ice During Shipping.Remained Stable At 25°C For One Week Without Activity Lost. Can Be Stable After Several Times Of Freeze-thaw.||Cat.No:||REK08|
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Storage temperature: -20°C or below
Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain
Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
|Source||Recombinant E. coli|
|Appearance||Clear,colorless to yellowish liquid|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||25.8 ±2.6 kDa|
UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.
4. STABILITY OF STORAGE AND TRANSPORT
(1)Stability of storage: Recombinant enterokinase should be stored under -20°C in sealed container.It is stable within 24 months.If stored at 25°C ,it is stable within one week.It is no activity loss after 5 times repeated freezing and thawing.
(2)Stability of transport: The product is stable by blue ice insulation transport.
Contact Person: Miss Eland
Source: E. Coli
M.W.: Theoretical MW: 25,850 Da;The apparent MW on SDS-PAGE: about 27,000 Da
Storage: Store at -20°C after delivery.
Specific activity: One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in a buffer 25mMTris-HCl, pH 8.0.Substrate: a special fusion protein.