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Shanghai Yaxin Biotechnology Co.,Ltd.

Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery

    • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery
    • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery
    • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery
    • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery
    • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery
  • Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery

    Product Details:

    Place of Origin: China
    Brand Name: YaxinBio
    Certification: NSF ISO 9001 2015
    Model Number: REK08

    Payment & Shipping Terms:

    Minimum Order Quantity: 100U
    Price: 65$/100u
    Packaging Details: ice packaging
    Payment Terms: T/T
    Supply Ability: 1000ku per week
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    Detailed Product Description
    Source: E. Coli M.W.: Theoretical MW: 25,850 Da;The Apparent MW On SDS-PAGE: About 27,000 Da
    Storage: Store At -20°C After Delivery. Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.
    Stability: Keep Cool With Blue Ice During Shipping.Remained Stable At 25°C For One Week Without Activity Lost. Can Be Stable After Several Times Of Freeze-thaw. Cat.No: REK08

    Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery

     

    Recombinant Enterokinase

     

    Catalog Number:REK08

    CAS:9017-74-8
    EC:3.4.21.9

    Storage temperature: -20°C or below

    Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain

     

    1.Description

     

    Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.

     

     

    2.MAIN FEATURES

     

    Source Recombinant E. coli
    Appearance Clear,colorless to yellowish liquid
    Activity ≥5 u/μl
    Purity(SDS-PAGE) Single major band
    Molecular Weight(SDS-PAGE) 25.8 ±2.6 kDa

    UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

     

    3.Advantages

    1. Animal origin free: Recombinant enterokinase is no exogenous virus contamination,and any animal origin material is not used in the production process.
    2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
    3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
    4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
    5. Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
    6. Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

    4. STABILITY OF STORAGE AND TRANSPORT

    (1)Stability of storage: Recombinant enterokinase should be stored under -20°C in sealed container.It is stable within 24 months.If stored at 25°C ,it is stable within one week.It is no activity loss after 5 times repeated freezing and thawing.

    (2)Stability of transport: The product is stable by blue ice insulation transport.

     


     

     

     

     

    Recombinant Enterokinase expressed by E.coli and can Store at -20°C after delivery

     

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

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