Home ProductsSequencing Grade Trypsin

Recombinant Endoproteinase Lys-C

Recombinant Endoproteinase Lys-C

    • Recombinant Endoproteinase Lys-C
  • Recombinant Endoproteinase Lys-C

    Product Details:

    Place of Origin: Xuhui, Shanghai, China
    Brand Name: Yaxinbio
    Certification: NSF ISO 9001:2015
    Model Number: RLC18

    Payment & Shipping Terms:

    Minimum Order Quantity: 1g
    Packaging Details: 100ug, 1mg; ice packing, carton
    Payment Terms: T/T, Western Union, MoneyGram
    Supply Ability: 99999
    Contact Now
    Detailed Product Description

    Recombinant Endoproteinase Lys-C

     

    Catalog Number: RLC18
    EC: 3.4.21.50

    Storage temperature: -20℃ or below, seal, dark

    Source: Recombinant Endoproteinase Lys-C is a genetically engineered protein expressed in E.coli

    Protein Sequence:The amino acid sequence of recombinant endoproteinase Lys-C is similar to Lys-C from achromobacterial proteasel

     

    1.DESCRIPTION

    This product is a Recombinant Endoproteinase Lys-C, expressed in E. Coli with the similar sequence & proteolytic activity of Lys-C from Achromobacterial proteasel (API). Due to its recombinant form there is no other unwanted serine protease such as Lys-N, Arg-C and only lysine-specific.

     

    2.MAIN FEATURES

    Appearance: white or pale white lyophilized powder packed in 0.1mg, 1.0mg & 10mg/vial (according to actual rLys-c content))

    Content assay: Lowry Method (labeled ±10%)

    SDS-PAGE: Clear single strap, scan purity more than 95%; relative molecular weight is 31±3.1 KDa

    RP-HPLC purity: the main peak more than 95%

    Enzyme assay activity: ≥ 3.0 AU/mg

    Definition of activity: One Amidase Unit (AU) is the amount of enzyme, which produces 1 micromole Ac-Lys-pNA per minute at 25 mmol/L Tris-HCl buffer pH 9.0 at 37°C.

     

    3.RECOMMEND USAGE

    Ratio of Lys-C & target protein is 1:1000-1:20000; pH 7.0-9.5, reaction temperature 30-37℃, 2-24 hr.

    Optimization by adjusting the temperature, time & enzyme ratio can achieve specific purpose.

    Enzyme inhibitors such as TLCK, Guanidine Hydrochloride should not be used

    Existence of 0.2% SDS, 1.0% Triton, 6 mol/L Urea, 0.5 mol/L NaCl, 20% Acetonitrile in reaction buffer does not reduce the activity of Lys-C enzymatic digestion.

     

    4.STABILITY OF STORAGE AND TRANSPORT

    Stability of storage: It is lyophilized powder and is stable to use for at least 1 year with storage under -20℃ or below. Reconstituted with double distilled water under 2-8℃ its stability can last for 7 days. It is recommended to dilute the designated concentration by 25mol/L Tris-HCl (pH 8.5). Avoid repeated freeze-thaw after being dissolved.

    Stability of transport: The product is stable by blue ice insulation transport.

     

    5.PRODUCT ADVANTAGE

    Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.

    Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

    High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

    Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

     

    requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

    Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

     

    6.ANALYSIS of PURITY CONFIRMATION

    SDS-PAGE Electrophoresis

    Recombinant Endoproteinase Lys-C 0

    RP-HPLC analysis

    Recombinant Endoproteinase Lys-C 1

    7.RELATED PRODUCTS

    Recombinant carboxypeptidase B

    Sequencing grade trypsin

    Recombinant trypsin

    Recombinant enterokinase

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

    Send your inquiry directly to us (0 / 3000)

    Best Products
    Other Products