|Place of Origin:||China|
|Certification:||NSF ISO 9001 2015|
|Model Number:||Recombinant trypsin solution|
|Minimum Order Quantity:||100ml|
|Refold:||Chromatography||Purify（HPLC）:||NLT 70% β-trysin, NMT 20% α-trypsin|
|Name:||Recombinant Trypsin Solution||Action:||Cell Dissociation|
Recombinant trypsin solution refolded and purified by chromatography , cell dissociation solution
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSFsoybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.
Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.
The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
|Recombinant Trypsin Solution||RTS04||2000 BAEE u/ml||100 ml,500ml||YaxinBio|
UNIT DEFINITION:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃ (1cm light path). 1 USP unit = 3 BAEE unit.
Contact Person: Miss Eland