|Place of Origin:||Shanghai, China|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||100ml|
|Packaging Details:||100ml, 500ml; ice packing, carton|
|EDTA:||Without EDTA||Formula With:||Human Trypsin|
|Source:||E.coli||Name:||Recombinant Trypsin Solution|
trypsin cell culture
Human Trypsin, Recombinant Trypsin Solution, No EDTA
Recombinant Trypsin Solution
Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.
YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is 7.0-11.0.
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥3800 USP units/mg pro.|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||24.0±2.4 kDa|
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.
Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.
Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.
The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
Contact Person: Miss Eland