|Place of Origin:||China|
|Certification:||NSF ISO 9001 2015|
|Minimum Order Quantity:||100ml|
|Name:||Recombinant Trypsin Solution||Solution:||Cell Dissociation Solution|
Cell Dissociation solution , recombinant trypsin solution optimized for cell dissociation
Recombinant Trypsin Solution
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues.Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography.
FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.
Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.
The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
METHOD OF USE
Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.
Step 2. Dissolve the trypsin powder.
Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.
Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.
Note 2. If necessary, after dissolving, immediately dispense into suitable
containers, then stored under -20℃. To use it, dissolve it at room temperature.
Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.
Contact Person: Miss Eland