|Place of Origin:||China|
|Certification:||NSF ISO 9001 2015|
|Model Number:||V8 Protease|
|Minimum Order Quantity:||50ug|
|Delivery Time:||5-8 work days|
|Name:||Endoproteinase Glu-C||Species:||Staphylococcal Serine Proteinase|
|Band:||Yaxin||Specific Activity:||≥ 5.0 U /mg Pro.|
|Purity(SDS-PAGE):||Single Major Band|
V8 Protease, also known as Endoproteinase Glu C, is a staphylococcal serine proteinase
Sequencing Grade V8 Protease
Full Name:Sequencing grade recombinant V8 protease;Staphylococcus aureus strain V8
Synonyms:V8 protease;Endoproteinase Glu-C;Endoproteinase Asp-C
Storage temperature: 2-8°C
Source: Recombinant V8 protease is a genetically engineered protein expressed in E.coli
Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK).
Approx.5U/mg at 25℃ with Z-Phe-Leu-Glu-4-nitranilide as the substrate(approx.500U/mg at 37℃ with casein as the substrate).
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant V8 protease should be stored under 2-8°C in sealed container.It is stable within 6 months after dissolved with1mM HCl and 50mM HAC at -20°C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C 12h. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
Contact Person: Miss Eland