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| Place of Origin: | Shanghai China |
| Brand Name: | yaxinbio |
| Certification: | NSF ISO 9001 2015 |
| Model Number: | SRCT10 |
| Minimum Order Quantity: | 100ug |
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| Packaging Details: | 100ug、1mg |
| Source: | E. Coli | Specific Activity: | ≥ 1500 Units/mg Pro |
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| SDS-PAGE (MW): | 24.0±2.4 KDa | Purity(HPLC): | ≥ 95% |
| Suitable: | Digestion Reactions In-solution Or In-gel. | Optimal PH: | 7.0–9.0 |
| Brand: | Yaxinbio |
The sequencing grade human chymotrypsin can be used alone or in combination with other proteases
Sequencing Grade Human Chymotrypsin
Catalog Number:SRCT10
CAS: 9004-07-3
EC: 3.4.21.1
Storage temperature: 2-8℃
Source: Sequencing grade chymotrypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of sequencing grade chymotrypsin is identical to human chymotrypsin
DESCRIPTION
Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0.
MAIN FEATURES
| Source | Recombinant E. coli |
| Appearance | White or off white powder |
| Specific activity | ≥1500 USP units/mg pro. |
| SDS-PAGE | Single major band |
| SDS-PAGE (MW) | 24.0±2.4 kDa |
| Purity(HPLC) | ≥ 95% |
APPLICATION
1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
PRODUCT ADVANTAGE
Sequence grade recombinant protein: high specificity,good stability.
Animal origin free: Sequencing grade chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
PRODUCT USAGE
Sequencing grade chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
Contact Person: Miss Eland
