NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC

Sequencing grade human chymotrypsin a recombinant serine endopeptidase expressed in E.coli and purified with HPLC

DESCRIPTION

Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues:Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0. 

MAIN FEATURES

APPLICATION

1)Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

2)This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

RECOMMEND USAGE

For disolving buffer ,it is 50mMHAC,to get the terminal concentration of 0.5μg/μl,used once time or stored below -20°C.For the digestion buffer,in general,it is 100mM NH₄HCO₃ containing 5mM CaCl_{2 ,}at 30°C.to dissolve the aim protein and then added chymotrypsin (in general,W:W=1:20-1:100) .Self digestion may occur if temperatures above 37°C.

STORAGE INSTRUCTIONS

Recombinant Chymotrypsin lyophilized should be stored under 2°C-8°C in sealed container. It is stable within 24 months.After dissolved,it should be stored under -20°C.It is stable within 24 months and above 90% activity remained after 3 times repeated freezing and thawing.