Home ProductsSequencing Grade Chymotrypsin

NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC

NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC

    • NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC
    • NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC
    • NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC
    • NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC
  • NSF Sequencing Grade Chymotrypsin , Recombinant Serine Endopeptidase Expressed In e.Coli And Purified With HPLC

    Product Details:

    Place of Origin: China
    Brand Name: yaxinbio
    Certification: NSF ISO 9001 2015
    Model Number: SRCT10

    Payment & Shipping Terms:

    Minimum Order Quantity: 100ug
    Packaging Details: 100ug
    Contact Now
    Detailed Product Description
    Source: Recombinant E.coli Format: Lyophilized
    Appearance: White Or Off White Powder Specific Activity: >=1500 USP Units/mg Pro.
    SDS-PAGE: Single Major Band Purity(HPLC): >= 95%

    Sequencing grade human chymotrypsin a recombinant serine endopeptidase expressed in E.coli and purified with HPLC

     

     

    DESCRIPTION

    Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues:Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0. 

     

     

    MAIN FEATURES

    Source Recombinant E. coli
    Appearance White or off white powder
    Specific activity >=1500 USP units/mg pro.
    SDS-PAGE Single major band
    SDS-PAGE (MW) 24.0±2.4 kDa
    Purity(HPLC) >= 95%
    Format Lyophilized

     

     

    APPLICATION

    1)Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

    2)This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

     

     

    RECOMMEND USAGE

    For disolving buffer ,it is 50mMHAC,to get the terminal concentration of 0.5μg/μl,used once time or stored below -20°C.For the digestion buffer,in general,it is 100mM NH4HCO3 containing 5mM CaCl2 ,at 30°C.to dissolve the aim protein and then added chymotrypsin (in general,W:W=1:20-1:100) .Self digestion may occur if temperatures above 37°C.

     

     

    STORAGE INSTRUCTIONS

    Recombinant Chymotrypsin lyophilized should be stored under 2°C-8°C in sealed container. It is stable within 24 months.After dissolved,it should be stored under -20°C.It is stable within 24 months and above 90% activity remained after 3 times repeated freezing and thawing.

    Contact Details
    Shanghai Yaxin Biotechnology Co.,Ltd.

    Contact Person: Miss Eland

    Send your inquiry directly to us (0 / 3000)

    Other Products