Home ProductsSequencing Grade Trypsin

Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein

Certification
China Shanghai Yaxin Biotechnology Co.,Ltd. certification
China Shanghai Yaxin Biotechnology Co.,Ltd. certification
Although we are first cooperation, your company left a very deep impression on me. You are a very honest company.

—— Jim Holf

Thank you very much. Your products are very good. Next time I will continue to buy. It is worth to recommend the company.

—— Susan Vird

I'm Online Chat Now

Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein

Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein
Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein

Large Image :  Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein

Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging
Payment Terms: T/T
Supply Ability: 1000g per week
Detailed Product Description
Product: Sequencing Grade Trypsin Cat. No.: SRT0201
Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
High Light:

sequencing grade modified trypsin

,

trypsin endopeptidase

Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein

 

sequencing grade trypsin

DESCRIPTION

 

YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features

 

Source

E. Coli

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Specific activity

≥ 4500 USP units/mg pro

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

 

 

 

Unit Definition

 

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Application

 

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

 

Recommend Usage

 

1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

2. When using this product, thaw it at room temperature, and mix gently before use.

3. No activity lost when freeze-thaw 5 cycles.

 

Storage Instructions

 

1. The solution should be stored under -70℃, It is stable within 24 months.

2. Above 95% activity remained after 5 times repeated freezing and thawing.

3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

Endoproteinase Llys-c Sequencing Grade for Digesting Specific Protein 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

Send your inquiry directly to us (0 / 3000)