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YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
  • YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 175$/100mg
Packaging Details: ice packaging
Delivery Time: 7 work days
Payment Terms: T/T in advance
Supply Ability: 100000g per year
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Detailed Product Description
Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
Specific Activity: ≥170 U/mg Pro Physical Form: White Or White-like Lyophilized Powder
Additives: Tris Salts, NaCl Salts And Carbohydrates Protein Content: 35% ~ 50%
High Light:

trypsin endopeptidase

,

carboxypeptidase enzyme

YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

 

Recombinant Carboxypeptidase B

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

 

Main Features

 

Source

E. Coli

Purified by

HPLC

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.

 

 

 

 

 

 

 

Unit  Definition

 

One Unit of carboxypeptidase B activity hydrolyzes one micromole of  hippuryl-L-arginine per minute at 25℃, pH 7.65.

 
Applications
 
Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

 

Recommend  Usage

 

To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 
Pathway
 
Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Protein digestion and absorption, organism-specific biosystem
 
Function
 
carboxypeptidase activity;
metallocarboxypeptidase activity;
zinc ion binding
YaxinBio a Supplier of  Recombinant Carboxypeptidase B expressed  in E.coli 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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