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Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

  • Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
  • Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
  • Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
  • Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RHT03
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 89$/10mg
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Human Trypsin 2 Packaging: 10mg,100mg Or Bulk
Specific Activity: ≥2500 USP U/mg Pro. Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, And Other Protease Contaminant.
Related Product: Recombinant Porcine Trypsin Manufacturer: YaxinBio
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trypsin digestion

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stem cell culture

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

 

Recombinant Human Trypsin

 

Description

 

1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
2.Recombinant human trypsin is  protein expressed in E.coli through a genetically engineered and purified by high pressure liquid chromatography.  Enzyme activities such as carboxypeptidase A and chymotrypsin are not contained in the trypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

 

 

 

 

 

 

 

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white powder
Specific activity ≥2500 USP units/mg pro.
Protein Content ≥ 90%
Purity(HPLC) ≥ 95%

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

Advantages

Animal origin free:The use of recombinant human trypsin eliminates the risk of virus presence and any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio recombinant human trypsin belongs to the AOF level 3.

Recombinant human trypsin:The amino acid sequence is the same as the human trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

Recombinant human trypsin provides increased specificity and eliminates

contaminating activities found in lower purity enzymes.

No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Purity is more than 95% by HPLC.

 

 

 

 

 

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin 0

 

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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