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Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

  • Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
  • Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
  • Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
  • Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000gper week
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Detailed Product Description
Product: Sequencing Grade Trypsin Cat. No.: SRT0201
Packaging: 100ug 1mg Manufacturer: YaxinBio
Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
High Light:

sequencing grade modified trypsin

,

serine protease

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

 

Sequencing Grade Trypsin

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features

 

Source

E. Coli

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Specific activity

≥ 4500 USP units/mg pro

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

 

 

Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Application

 

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

 

Recommend Usage

 

1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

2. When using this product, thaw it at room temperature, and mix gently before use.

3. No activity lost when freeze-thaw 5 cycles.

 

Storage Instructions

 

1. The solution should be stored under -70℃, It is stable within 24 months.

2. Above 95% activity remained after 5 times repeated freezing and thawing.

3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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