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Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli

Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli

  • Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli
  • Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli
  • Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli
  • Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli
Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 98$/100ug
Packaging Details: ice packaging, carton
Delivery Time: 7 days
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Source: E. Coli Purified By: HPLC
Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
High Light:

trypsin endopeptidase

,

serine protease

Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli

 

Sequencing Grade Trypsin

 

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features

 

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

Reconstitution

 

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

 

Specificity

 

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Sequencing Grade Trypsin, Purified by HPLC, Expressed in E.coli 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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