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Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

  • Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
  • Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
  • Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
  • Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
  • Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 1mg
Price: 385$/mg
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Trypsin Source: E. Coli
Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
Purity: One Single Main Band At 24kD By SDS-PAGE RELATED PRODUCT: Recombinant Trypsin
High Light:

trypsin endopeptidase

,

serine protease

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

 

Sequencing Grade trypsin

 

Catalog Number:SRT0202

CAS:9002-07-7
EC:3.4.21.4

Source:Expressed in E. Coli, methylation modified.

 

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

 

Application

 

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

 

General description

 

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

 

PRODUCT USAGE

 

The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

 

Preparation Note

 

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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