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Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis

Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis

  • Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
  • Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
  • Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
  • Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
  • Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Trypsin Cat. No.: SRT0201
Physical Form: Lyophilized Powder Specific Activity: >4500 USP Units/mg Pro
Purity: One Single Main Band At 24kD By SDS-PAGE Contaminant Activity: No Chymotrypsin, Carboxypeptidase A, Or Other Protease Contaminant.
High Light:

trypsin endopeptidase

,

serine protease

Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis

 

Sequencing Grade Trypsin

 

Catalog Number:SRT0201

CAS:9002-07-7
EC:3.4.21.4

Storage temperature: 2-8℃

Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli

Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin

 

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features 

 

Source

E. Coli

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

 

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

Sequencing Grade Recombinant Trypsin, For Mass Spectrometric Analysis 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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