Home ProductsSequencing Grade Trypsin

Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio

Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio

  • Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
  • Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
  • Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
  • Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
  • Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
Contact Now
Detailed Product Description
Product: Sequencing Grade Trypsin Cat. No.: 9002-07-7
Packaging: 100ug 1mg Manufacturer: YaxinBio
Specific Activity: ≥4500 USP /mg Pro RELATED PRODUCT: Recombinant Carboxypeptidase B
High Light:

trypsin endopeptidase

,

serine protease

Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio

 

Sequencing Grade Trypsin

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere

with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin

is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

RECOMMEND USAGE

It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

 

STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

Main Features

 

Source

E. Coli

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

 

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a 

reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

Sequencing Grade Recombinant Trypsin, for in-gel Protein Digestion by Shanghai YaxinBio 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

Send your inquiry directly to us (0 / 3000)

Best Products
Other Products