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Sequencing Grade Modified Trypsin, For Protein Identification

Sequencing Grade Modified Trypsin, For Protein Identification

  • Sequencing Grade Modified Trypsin, For Protein Identification
  • Sequencing Grade Modified Trypsin, For Protein Identification
  • Sequencing Grade Modified Trypsin, For Protein Identification
  • Sequencing Grade Modified Trypsin, For Protein Identification
  • Sequencing Grade Modified Trypsin, For Protein Identification
Sequencing Grade Modified Trypsin, For Protein Identification
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRT0201
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Trypsin Manufacturer: YaxinBio
Purified By: HPLC Specific Activity: NLT 4500 USP U/mg Pro
Purity(HPLC): ≥95% SDS-PAGE: Single Major Band
High Light:

sequencing grade modified trypsin

,

trypsin endopeptidase

Sequencing Grade Modified Trypsin, For Protein Identification

 

Sequencing Grade Trypsin

Storage temperature: 2-8℃

Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli

Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin

 

DESCRIPTION

YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

RECOMMEND USAGE

It is recommended to dissolve or dilute with 50 mM HAc or 1 mM HCl. When used, dilute in 50 mM NH4HCO3 or ph7.0-8.0 buffer solution. 1 mM CaCl2 is recommended In the digestion buffer. The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-8.0.

 

STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

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Sequencing Grade Modified Trypsin, For Protein Identification 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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