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Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

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Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution Sequencing Grade Chymotrypsin for Digestion Reactions In-solution Sequencing Grade Chymotrypsin for Digestion Reactions In-solution Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Large Image :  Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCT03
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 158$/100ug
Packaging Details: dry ice
Delivery Time: 7 days
Payment Terms: T/T in advance
Supply Ability: 1000g per week
Detailed Product Description
Source: E.Coli Purity: NLT 95% By HPLC Analysis.
Physical Form: Lyophilized Powder Specific Activity: NLT 1500 USP Units/mg Pro
Contaminant Activity: No Carboxypeptidase A, Or Other Proteases Contaminant. CAS: 9004-07-3
High Light:

sequencing grade modified trypsin

,

serine protease

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

 

 

Descirption

 

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

CAS: 9004-07-3
EC: 3.4.21.1

Source: human chymotrypsin, expressed in E. Coli.

 

Main Features

 

Source

E. Coli

M.W.

26,950 Da  

Purity

≥ 98 % by HPLC.

Format

Lyophilized

Specific activity

≥ 3000 units/mg pro

 

Application

 

1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

 

Recommend Usage

 

For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.

 

Storage Instructions

 

Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

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