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Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry

Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry

  • Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
  • Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
  • Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
  • Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
  • Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RPT0201
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 65$/10mg
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Porcine Trypsin Physical Form: White Lyophilized Powder
Specific Activity: ≥3800 USP U/mg Pro Purity: > 99%
Related Product: Recombinant Protein A
High Light:

porcine trypsin

,

cell culture trypsin

Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry

 

Recombinant Trypsin

 

Description

 

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end

of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF and by metal chelating agents, e.g.EDTA.

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

Advantages

 

Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white, or yellowish powder
Specific activity ≥3800 USP units/mg pro.
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Recommend Usage

 

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

Storage Instructions

 

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

above 90% activity remained after 10 times repeated freezing and thawing.

Recombinant Trypsin, Lyophilized Powder, Trypsin for Mass Spectrometry 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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