Home ProductsTrypsin Solution

Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation

Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation

  • Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation
  • Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation
  • Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation
  • Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation
Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RTS04
Payment & Shipping Terms:
Minimum Order Quantity: 100ml
Price: consult
Packaging Details: ice packaging
Payment Terms: T/T in advance
Supply Ability: 10000L per week
Contact Now
Detailed Product Description
Product: Recombinant Trypsin Solution Cat. No.: RTS04
Specific Activity: 2000 BAEE U/ml Packaging: 100ml, 500ml, Bulk
Convenient: Dissociate Adherent Cells RELATED PRODUCT: Recombinant (Porcine/Human )Trypsin
High Light:

trypsin for cell culture

,

porcine trypsin

Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation

 

 

Description

 

Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin  expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

 

Features And Benefits

 

Recombinant trypsin

Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin

inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.

 

Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform

to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

 

Advanges

 

High safety The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
High purity

Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

Good work without EDTA No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.
Convenient

Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

 

 

 

 

 

 

 

 

 

 

 

Unit Definition:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in

a reaction volume of 3.2ml at pH7.6 and 25℃ (1cm light path). 1 USP unit = 3 BAEE unit

 

Instructions for use

 

The following instructions are applicable for most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines (see notes below).

1. Wash the cells with DPBS w/o Ca, Mg (Cat No. 02-023-1).

2. Add 1ml of Recombinant Trypsin Solution to each T-25 tissue culture flask.

3. Incubate the flask at 37°C for 2 min or longer as necessary. Verify cell’s detachment using inverted microscope.

4. When cells are completely detached, add 5-10 ml of culture medium (MSC NutriStem® XF, Cat. No. 05-200-1). Alternatively use diluted (1:50, in DPBS) Soybean Trypsin Inhibitor (SBTI, Cat. No. 03-048-1).

5. Pellet the cells by centrifugation, and discard the supernatant.

6. Resuspend the cells in growth medium, and seed as desired.

 

Recombinant Trypsin for Cell Separation and Adherent Cells Dissociation 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

Send your inquiry directly to us (0 / 3000)

Other Products