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Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified

Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified

  • Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
  • Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
  • Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
  • Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
  • Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: 3.4.21.61
Payment & Shipping Terms:
Minimum Order Quantity: 50U
Price: $2000
Packaging Details: 50ug、1mg、5mg
Delivery Time: 7 work days
Payment Terms: T/T in advance
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Detailed Product Description
Purified By: HPLC Mol.weight: 67 KD
Specific Activity: ≥10u/mg Source: Pichia Pastoris
12%SDS-PAGE: Single Main Stripe Temperature: -20 °C Or Below.
High Light:

Recombinant protease

,

saccharomyces cerevisiae yeast

Recombinant Kex2 Protease Pichia Pastoris Source with HPLC Purified

 

Recombinant Kex2 Protease

 

 

Main Features

 

Source: Pichia pastoris

Specific activity: ≥10u/mg

12.5%SDS-PAGE: Single main stripe

Mol.weight: 67±6.7 kDa

 


Recommend storage buffer:pH5.0-5.5 20mM NaAc-HAc buffer and 2mM Ca2+.

Recommend reaction buffer:pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+ .

To prepare 1-10mg/ml Kex2 solution with 20mM NaAc-HAc (pH 5.2) and 2mM Ca2+ for a stored solution, and diluted with reaction buffer shch as pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+. If used after dissolved immediately, with the reaction buffer pH 7.0-9.0 50mM Tris-HCl or HEPES, 5mM Ca2+ to dissolve directly.

 

DESCRIPTION

Kex2 is a Ca2+ dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α-factor and killer-toxin precursors maturing.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 protease is a genetically engineered protein expressed in Pichia pastoris and with equivalent properties compared to native Saccharomyces cerevisiae Kex2. The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF,TPCK,TLCK inhibition.The optimal pH of Kex2 protease is 9.0. It is stable in buffer (pH 5.0-6.0).

 

PRODUCT ADVANTAGE

Animal origin free:Recombinant Kex2 protease is no exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

Lyophilized powder: The product is lyophilized powder and is easy to store and transport.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

Product Infomation

 

Product Specific    Recombinant Kex2 Protease

activity                   ≥10u/mg

Packaging             50µg/each

Manufacturer         YaxinBio

   

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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