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Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade

Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade

  • Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
  • Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
  • Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
  • Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
  • Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 150$/10mg
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Carboxypeptidase B Brand: YaxinBio
Protein Content: 35% ~ 50% Specific Activity: ≥170 Units/mg Pro
Purity: > 95% Cat. No.: RCPB01
High Light:

sequencing grade modified trypsin

,

carboxypeptidase enzyme

Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade

 

Recombinant Carboxypeptidase B

 

CAS: 9025-24-5
EC: 3.4.17.2

Source: Expressed in E. Coli.

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

Main Features

 

Source E. Coli
Purified by HPLC
Format White or white-like or yellowish lyophilized
Additives Tris salts, NaCl salts and carbohydrates
Purity Single main band on SDS-PAGE
M.W. 33.8kD
Contaminant activity Less than 10 ppm of recombinant trypsin.

 

 

 

 

 

 

 

 

 

Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

 

Advantages

 

Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.

 

 

Recommend Usage

 

To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 

Recombinant Enzyme, For Protein Digestion, Carboxypeptidase B, Proteomics Grade 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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