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Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.

Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.

  • Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.
  • Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.
  • Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.
  • Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.
Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 67$/100mg
Packaging Details: ice packaging, carton
Delivery Time: 7 work days
Payment Terms: T/T in advance
Supply Ability: 100g per week
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Detailed Product Description
Product: Recombinant Carboxypeptidase B Cat. No.: RCPB01
Source : E. Coli Purified by: HPLC
Protein conten: 35% ~ 50% Contaminant activity: No Chymotrypsin,carboxypeptidase A,and Other Protease Contaminant.Less Than 10ppm Of Recombinant Trypsin.
High Light:

sequencing grade modified trypsin

,

carboxypeptidase enzyme

Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro.

 

Recombinant Carboxypeptidase B

 

Strain: Rat
Form:Lyophilized from 20 mM Tris, pH 8.0 + 50 mM NaCl.
Enzyme Commission Number: 3.4.17.2
Bio-activity:50-55 units/mg protein carboxypeptidase B
CAS No.:9025-24-5

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

 

Advantages

 

Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

 

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

 

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.

 

Main Features

 

Format

White or white-like or yellowish lyophilized

Additives

Tris salts, NaCl salts and carbohydrates

Specific activity

 ≥170 units/mg pro.

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.

 

 

 

 

 

 

 

 

Unit Definition

 

One Unit of carboxypeptidase B activity hydrolyzes one micromole of  hippuryl-L-arginine per minute at 25℃, pH 7.65.

 

Recommend Usage

 

To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

 
Applications
 
Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.
 

Recombinant Enzyme, Carboxypeptidase B, High Purity, 170 U/mg pro. 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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