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Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity

Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity

  • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
  • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
  • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
  • Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Payment & Shipping Terms:
Minimum Order Quantity: 100U
Price: 65$/100u
Packaging Details: ice packaging, carton
Delivery Time: 6 days
Payment Terms: T/T in advance
Supply Ability: 1000ku per week
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Detailed Product Description
Product: Recombinant Enterokinase Source: E.Coli
Packaging: 100U, 1KU Or Bulk Fusion Protein Concentration: ≥5 U/μl
Storage: Store At -20°C After Delivery. RELATED PRODUCT: Sequencing Grade Trypsin
High Light:

enterokinase enzyme

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recombinant enzymes

Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity
     

Recombinant Enterokinase 

 

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Storage temperature: -20°C or below

Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain

 

Description

 

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme

has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic

acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or

tags to get aim protein with native amino acids sequence.

 

Advantages

 

1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-

Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.

 

Recommend Usage Condition:

 

Cutting condition 25mM Tris-HCl 8.0
Fusion protein concentration 0.1-1mg/ml (total protein content: 0.5-1.0mg)
EK content 1-2U
Temperature 25℃
Time overnight or 16h-24h for digestion

 

 

 

 

 

 

 

Biochemphysiol Actions

 

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen

to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer, wherein, the light and the heavy chains

are linked by two disulfide bridges. Native enterokinase is composed of an 800 amino acid heavy

chain and a 235 amino acid light chain. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. This cleavage site is incorporated into the FLAG tag.

 

Physical Form


supplied as a solution in 20 mM Tris-HCl, 200 mM NaCl, and 50% glycerol

 

Application

 

Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic

activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential

activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used

in a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.

 

Recombinant Enterokinase, Genetically Engineered Protein, High Specificity and High Purity 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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