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Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase

Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase

  • Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
  • Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
  • Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
  • Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
  • Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Payment & Shipping Terms:
Minimum Order Quantity: 100U
Price: 65$/100u
Packaging Details: 100U, 500U, 1000U, 10KU, 100KU, 1MU; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000ku per week
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Detailed Product Description
Product: Recombinant Enterokinase Source: E.Coli
Specific Activity: ≥1 U/μl;≥5 U/μl CAS: 9017-74-8
EC: 3.4.21.9 RELATED PRODUCT: Sequencing Grade Chymotrypsin
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enterokinase enzyme

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Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase

 

Description

 

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

 

Advantages

 

1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (D-D-D-D-K)

2) No any other contaminated proteases, no non-specific cutting sites.

Recommend Usage Condition:

Cutting condition:             given an example: 25mM Tris-HCl 8.0

Fusion protein concentration:  0.1-1mg/ml (total protein content: 0.5-1.0mg)

EK content:                   1-2U

Temperature:                  25℃

Time:                         overnight or 16h-24h for digestion.

 

Main Features

 

M.W.

25,850 Da  

Specific Activity

One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein. 

Storage Temperature

-20°C or below.  

Stability

Keep cool with blue ice during shipping. Remained stable at 25°C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.

 

 

 

 

 

 

 

 

 

 

Common Components Influence the Action of Enterokinase

 

>200mM imidazole or >200mM NaCl or >5%glycerin, the reaction may be effected.The following suggestions are given:

1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.

2) If the dialysis is inconvenient, please dilute the sample to <100mM imidazole, <50mMNaCl, <5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).

3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.

 

Product Information

 

Product

 Activity

Packaging

Manufacturer

Recombinant Enterokinase

≥5 u/μl

100u;500u;1ku; 10ku

YaxinBio


 

 

 

Recombinant Enterokinas, CAS 9017-74-8, Enzyme, Enterokinase 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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