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Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics

Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics

  • Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
  • Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
  • Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
  • Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
  • Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 198$/100ug
Packaging Details: 1mg, 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000 per week
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Detailed Product Description
Manufacturer: YaxinBio Product: Recombinant Carboxypeptidase B
Source: E. Coli Specific Activity: ≥200 U/mg Pro
Purity: > 95% Contaminant Activity: No Chymotrypsin,carboxypeptidase A,and Other Protease Contaminant. Less Than 10 Ppm Of Recombinant Trypsin.
High Light:

trypsin endopeptidase

,

carboxypeptidase enzyme

Recombinant Carboxypeptidase B, Sequence grade, For Detecting Antibody Proteomics

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

Application

 

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.

 

RECOMMEND USAGE

 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.

 

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.

Recombinant Carboxypeptidase B, Sequence Grade, For Detecting Antibody Proteomics 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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