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Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade

Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade

  • Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
  • Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
  • Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
  • Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
  • Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: SRCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Sequencing Grade Carboxypeptidase B Manufacturer: YaxinBio
Source: E. Coli Purified By: HPLC
Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Human Trypsin
Source: Expressed In E.coli Impact: Proteomics Grade
High Light:

sequencing grade modified trypsin

,

trypsin endopeptidase

Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade

 

Recombinant Carboxypeptidase B

 

Description

 

Carboxypeptidase B is a metalloenzyme, which is widely used for commercial and research purposes. Commercially available CPB purified from porcine or bovine pancreas is very expensive, and is not totally free from other proteases. In order to express the rat proCPB in Pichia pastoris, total RNA extracted from SD rat pancreas cells was reversely transcripted to synthesize cDNA, and the proCPB ORF was synthesized by PCR. After digestion with Xho I and EcoR I , the fragment was inserted into pPIC9, and the recombinant plasmid was named as pPIC9-proCPB. By digestion with Sac I , the lined pPIC9-proCPB was transformed into Pichia pastoris strains GS115 with PEG1000 and integrated into their genomes. In the inducement of methanol, recombinant proCPB was successfully expressed in Pichia pastoris, and could be secreted into the supernatant in the culture.

 

Main Features

 

Purity by HPLC

≥ 95%

Format

Lyophilized

Specific activity

≥ 200 units/mg pro

Contaminant activity

No chymotrypsin, carboxypeptidase A, or other proteases contaminant.

 

 

 

 

 

 

 

Application

 

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.

 

Recommend Usage

 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to

aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable

within 24 months and no activity lose after 10 times repeated freezing and thawing.

Recombinant Carboxypeptidase B, Expressed in E.coli, Proteomics Grade 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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