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Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry

Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry

  • Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
  • Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
  • Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
  • Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
  • Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RCPB01
Payment & Shipping Terms:
Minimum Order Quantity: 100ug
Price: 198$/100ug
Packaging Details: ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Product: Recombinant Carboxypeptidase B Manufacturer: YaxinBio
Source: E. Coli Purified By: HPLC
Contaminant Activity: No Trypsin, Chymotrypsin, Carboxypeptidase A, Or Other Proteases Contaminant. RELATED PRODUCT: Recombinant Chymotrypsin (sequencing Grade);
High Light:

trypsin endopeptidase

,

carboxypeptidase enzyme

Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry

 

Recombinant Carboxypeptidase B

 

Description

 

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

 

Main Features

 

Purity: Greater than 95% as determined by SDS-PAGE.

Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

Bilogical Activity:170 units/mg protein.

 

Application

 

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.

 

Recommend Usage

 

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.

 

Storage Instructions

 

Stability:Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below under 2-8℃ in sealed container. For long term storage it is recommended to be stored under -20℃. Please prevent freeze-thaw cycles.

 

Recombinant Carboxypeptidase B, Expressed in E.coli, for Mass Spectrometry 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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