|Place of Origin:||Shanghai|
|Certification:||NSF ISO 9001:2015|
|Model Number:||Recombinant Carboxypeptidase B|
|Minimum Order Quantity:||1mg|
|Packaging Details:||1mg, 10mg, 100mg; ice packing, carton|
|Delivery Time:||2-3 work days|
|Payment Terms:||T/T in advance|
|SOURCE:||Recombinant E. Coli||Appearance:||White Or Off White, Or Yellowish Powder|
|Specific Activity:||≥170 Units/mg Pro.||Purity(SDS-PAGE):||Single Major Band|
|Molecular Weight(SDS-PAGE):||33.8±3.4 KDa||Cas:||9025-24-5|
|Source:||Recombinant Carboxypeptidase B Is A Genetically Engineered Protein Expressed In E.coli|
sequencing grade modified trypsin,
Recombinant Carboxypeptidase B, Catalyzes Hydrolysis of Basic Amino Acids Lysine, Arginine and Histidin
Recombinant Carboxypeptidase B
Recombinant Carboxypeptidase B
Storage temperature: 2-8℃
Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥170 units/mg pro|
|Purity(SDS-PAGE)||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
UNIT DEFINITION:One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.
Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved wth sterile water or 25mM Tris-HCl, it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
(1)Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
(3)High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
(5)Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
The production of recombinant insulin and its analogues; the quality test of recombinant antibodies; the determination of C-terminal amino acids of proteins; the production of other recombinant polypeptides; and the enzymatic synthesis of certain specific compounds.
Sequencing grade trypsin
Recombinant human trypsin
Contact Person: Miss Eland