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Recombinant Bovine Enterokinase Cleaving DDDDK

Recombinant Bovine Enterokinase Cleaving DDDDK

  • Recombinant Bovine Enterokinase Cleaving DDDDK
  • Recombinant Bovine Enterokinase Cleaving DDDDK
  • Recombinant Bovine Enterokinase Cleaving DDDDK
  • Recombinant Bovine Enterokinase Cleaving DDDDK
Recombinant Bovine Enterokinase Cleaving DDDDK
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: REK08
Payment & Shipping Terms:
Minimum Order Quantity: 100U
Price: 65$/100U
Packaging Details: ice packaging
Payment Terms: T/T in advance
Supply Ability: 1000ku per week
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Detailed Product Description
Source: E. Coli Time: Overnight Or 16h-24h For Digestion.
Cat.No: REK08 Specific Activity: One Unit Is Defined As The Amount Of Enzyme Needed To Cleave 0.5mg Of Fusion Protein In 12 To16 Hours To Get 95% Completion At 25°C In A Buffer 25mMTris-HCl, PH 8.0.Substrate: A Special Fusion Protein.
Fusion Protein Concentration: 0.1-1mg/ml (total Protein Content: 0.5-1.0mg) Cutting Condition: Given An Example: 25mM Tris-HCl 8.0
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bovine enterokinase

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enterokinase enzyme

Recombinant Bovine Enterokinase Cleaving DDDDK

 

Recombinant Bovine Enterokinase

CAS:9017-74-8
EC:3.4.21.9

Source: bovine enterokinase, expressed in E. Coli

 

Description

 

YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.

 

 

MAIN FEATURES

 

Source Recombinant E. coli
Appearance Clear,colorless to yellowish liquid
Activity ≥5 u/μl
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 25.8 ±2.6 kDa

UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to 16 hours to get 95% completion at 25°C in 25 mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

 

 

Advantages

 

Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)
No any other contaminated proteases, no non-specific cutting sites

 

 

 

 

 

Product Information

 

Product

 Activity

Packaging

Manufacturer

Recombinant Enterokinase

≥5 u/μl

100u;500u;1ku; 10ku

YaxinBio

 

 

 

 

Recombinant Bovine Enterokinase Cleaving DDDDK 0

 

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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