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Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A

Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A

  • Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
  • Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
  • Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
  • Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
  • Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RSPA05
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 79$/10mg
Packaging Details: 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Brand: Yaxinbio Substrate: Porous Polystyrene-divinylbenzene
Ligand: Recombinant Protein A Recommend Flow Rate: 300-700 Cm/h
DBC: At Least 300 Mg IgG/mL Media At 500 Cm/h Maximum Pressure: 1500 PSI
High Light:

SPA protein A

,

purified protein

Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A

 

 Recombinant Protein A

CAS:91932-65-9

Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.

                    

Application(1)

 

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

 

APPLICATION(2)

 

The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

 

MAIN FEATURES

Appearance White or off white powder
Solubility Clear liquid
Bioburden ≤ 1 cfu/ mg
SDS-PAGE Single major band
SDS-PAGE (MW) 25000±1000 Daltons
UV Spectrum E275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC) ≥ 95%

 

STABILITY OF STORAGE AND TRANSPORT

 

Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

After dissolved with pure water, it should be stored under -20℃.

 

Stability of transport: The product is stable by blue ice insulation transport.

 

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Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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