|Place of Origin:||Shanghai|
|Certification:||NSF ISO 9001:2015|
|Minimum Order Quantity:||10mg|
|Packaging Details:||10mg, 100mg, 1g; ice packaging, carton|
|Payment Terms:||T/T in advance|
|Supply Ability:||1000g per week|
|Ligand:||Recombinant Protein A||Recommend Flow Rate:||300-700 Cm/h|
|DBC:||At Least 300 Mg IgG/mL Media At 500 Cm/h||Maximum Pressure:||1500 PSI|
SPA protein A,
Protein A, Recombinant Protein A, Expressed in E.coli, Not Antibody Column Purified Protein A
Recombinant Protein A
Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli,.
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.
After dissolved with pure water, it should be stored under -20℃.
Stability of transport: The product is stable by blue ice insulation transport.
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Contact Person: Miss Eland