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Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
  • Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification
Product Details:
Place of Origin: Shanghai
Brand Name: YAXINBIO
Certification: NSF ISO 9001:2015
Model Number: RSPA05
Payment & Shipping Terms:
Minimum Order Quantity: 10mg
Price: 79$/10mg
Packaging Details: 10mg, 100mg, 1g; ice packaging, carton
Payment Terms: T/T in advance
Supply Ability: 1000g per week
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Detailed Product Description
Brand: Yaxinbio Substrate: Porous Polystyrene-divinylbenzene
Detergents: Alkaline CIP Solvents Storage: 20% Ethanol 2-8 ℃
PH: PH 2-10 Temperature Range: 2-8℃
High Light:

SPA protein A

,

purified protein

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification

 

 Recombinant protein A                   

 

Application

 

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

 

Appearance White or off white powder
Solubility Clear liquid
Bioburden ≤ 1 cfu/ mg
SDS-PAGE Single major band
SDS-PAGE (MW) 25000±1000 Daltons
UV Spectrum E275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC) ≥ 95%

 

PRODUCT ADVANTAGE

  • Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
  • SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
  • The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
  • Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
  • High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
  • Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
  • Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
  • Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

Storage Instructions

Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

 

Usage

For Research or Manufacturing Purpose Only. Not for Human.

Preparation of Protein A Column Monoclonal and Polyclonal Antibody Purification 0

Contact Details
Shanghai Yaxin Biotechnology Co.,Ltd.

Contact Person: Miss Eland

Tel: +8613482039151

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